Biomolecules

Chemical Analysis of Living Tissue

To study the chemical constituents of living tissues at first a sample of living tissue is taken in mortar and pestle along with  some Trichloroacetic acid and following procedure is followed :-

·        

All the organic compounds  from the living organism is called – BIOMOLECULES (NCERT)

·         Wet Weight – Whole tissue weight

·         Dry Weight- Dehydrated tissue weight

·         Water content = Wet Weight – Dry Weight

Cellular Pool

·         Collection of different types of biomolecules and other chemical entities present in the cell is called cellular pool

Contents of cellular pool

1.       Organic Compounds

§  Carbohydrate

§  Proteins

§  Lipids

§  Other organic molecules

2.       Inorganic Compounds

§  Water

§  Na

§  K

§  Ca

§  Mg

§  Sulphate ions

§  Phosphate ions

Average composition of cells

Water	70-90%
Proteins	10-15%
Carbohydrate	3%
Lipid	2%
Nucleic Acids	5-7%
Ions	1%

Biomolecules

·         Molecules of living origin

·         Can be classified in two types

1.       Micromolecules –

·         of simple structures

·         Mol. Wt. less than 1000da or between 18-800 da,

·         Present  in acid soluble pool

·         Used as monomers

2. Macromolecules –

·         Of complex structure

·         Mol.Wt. greater than 1000 da

·         Found in Acid insoluble pool

·         Normally polymers

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